سنة النشر

2021

العنوان

In-vitro and in-vivo therapeutic effects of vancomycin on methicillin-resistant staphylococcus aureus

الخلاصة

The present study aimed to evaluate the therapeutic influence of vancomycin on methicillin-resistant Staphylococcus aureus (MRSA) in-vitro and in-vivo. Thirty rabbits were divided equally into three groups; group A served as a negative control, group B serve as positive control given MRSA at 2.7 x 106 CFU/mL, group C infected with 2.7 × 106 CFU/mL MRSA isolates that treated by Vancomycin (40) mg/kg-S/C daily after two days of infection for five days. The results showed that different vancomycin concentrations different degrees of inhibition against areas MRSA were applied on media well diffusion. The inhibition zone scale was varied according to antibacterial agent concentration and, when the agent concentration increased, the inhibition zone increased proportionally. The gross examination was performed on all groups for five days post-infection. Subcutaneous injection with MRSA resulted in pus-filled lesions after 24 hours of infection with a mean diameter (5.7 cm3) in all infected groups following the lesions reaching maximum diameter (8.3 cm3) after10 days post-infection in the infected non-treated group. The treated group with vancomycin showed decreased lesion volume but not a significant difference compared with the untreated group after 48 hours of treatment. Gross inspection of sacrificed animals of infected untreated classes, five days after infection, showed shin area with MRSA with severe ulcerative pyogenic lesions and a significant increase in lesion volume. In conclusion, Vancomycin showed good activity against MRSA in-vivo and in-vitro. © 2021, Dr. Yashwant Research Labs Pvt. Ltd.. All rights reserved.